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The Role of Sanger Sequencing in COVID-19 Variant Detection
Covid-19 and Variants of Concern Since its first occurrence in 2019, COVID-19 has been spreading globally infecting close to 165 million people and having killed more than 3.4 million people as of May19, 2021. The CDC has recommended self-protection measures such as...
Quantification of Copy Number Proportions by Sanger Sequencing
Sanger sequencing data files can be used to measure copy number proportions A couple of months ago, we read the overlapping peaks of both the wildtype and the BRAF V600E mutant traces in the electropherogram of Sanger sequencing from a clinical sample. We wanted to...
GWAS and WES as Gene Discovery Tools for Parkinson Diseases
Parkinson Disease: a common neurodegenerative disorder Parkinson diseases (PD) is the second most common neurodegenerative disorder that usually has a late onset; it affects 1% of the population over age 60 and a total 4million people in the globe. The people with PD...
16s rRNA Gene Sequencing as the Gold-standard for Microbial Identification and Classification
Bacteria and pathogen identification and methods Microbes or pathogens identification is common for both research (microbial community studies) and clinics (pathogen identification for infectious diseases). In clinical microbiology labs, fast and correct...
NGS and Sanger Sequencing Go Hand in Hand for Genetic Analysis
SNPs and Human Diseases SNPs, single nucleotide polymorphism, is the simplest genetic variation and occurs in every 1000 bases in human genomic DNA. Among the 3.5 million SNPs, some lie in intergenic regions (non-coding regions that play regulatory roles) while others...
Fragment Analysis as a Standard Method for FLT3 Mutation Detection Endorsed by International Guidelines
FLT 3 and mutations affecting prognosis of acute myeloid leukemia FMS-like Tyrosine kinase 3 (FLT3) belongs to the class III tyrosine kinase family and is expressed on hematopoietic stem progenitor cells for cell survival and development. In acute myeloid leukemia...
Sanger Sequencing as the Routine Genotyping Method for Testing HIV Drug Resistance Mutations
Human immunodeficiency virus (HIV) type 1 is the major virus type that can lead to acquired immunodeficiency syndrome (AIDS). It is estimated that 37.9 millions of people are infected with HIV. Patients with HIV are currently treated with a combination of...
AdvancedSeq New Website Launch
AdvancedSeq Launches New Website Featuring cost-effective alternative products for Sanger Sequencing and Fragment Analysis[Pleasanton, California, November 5, 2019] AdvancedSeq, LLC announces the launch of their new website today featuring alternative products for...
Empower Your Service with Cost-effective Sanger Sequencing
Empower Your Service with Cost-effective Sanger SequencingThe Power of 40 years of Sanger Sequencing Sanger sequencing, the dideoxy chain-termination DNA sequencing method, is a mature technology that is still popular and relevant today. It first incorporates...
Is There a Cost-effective Alternative to BigDye®?
Is There a Cost-effective Alternative to BigDye®? Sanger Sequencing, the Gold standard for DNA Sequencing Sanger sequencing has been around for 40 years. As a gold standard for DNA sequencing, Sanger sequencing provides 99.9% accuracy and has been widely used for...
