ADS™ phi29 DNA Polymerase & RCA DNA Amplification Kit

phi29 DNA polymerase, derived from the Bacillus subtilis phage phi29, is a replicative polymerase with high processivity and strand-displacement activity. This enzyme has been widely used in modern molecular biology for various applications, including the amplification of plasmid templates for Sanger sequencing, viral genome sequencing using next-generation sequencing (NGS), genotyping, and whole-genome amplifications, such as the amplification of viral genomes or human genome DNA for NGS.

We provide phi29 DNA polymerase and the phi29 RCA DNA Amplification Kit. Both products can be used to amplify DNA at 30°C for 4 to 18 hours. The DNA template for amplification can be plasmid DNA, lysate from a single colony, or microliters of culture. The amplified product can be directly used as a Sanger sequencing template without further cleanup.

Screening for correct mutants using a single colony eliminates the need for growing cultures, as the DNA template is amplified by phi29 DNA polymerase within a few hours for Sanger sequencing. This allows for faster identification of mutants without the need for plasmid isolation.

Kit Components:

ADS™ phi29 DNA polymerase comes with the enzyme and a 10X buffer. The ADS™ phi29 RCA DNA Amplification Kit is a DNA amplification-ready kit that includes DNA lysate buffer, 2X Amplification Buffer, and phi29 DNA polymerase.

Using a PCR product as the template?

If you are using a PCR product as the template for sequencing, visit the PCR and PCR Cleaning Beads page.