ADS phi29 DNA Polymerase & RCA DNA Amplification Kit

phi29 DNA polymerase from the Bacillus subtilis phage phi29 is the replicative polymerase that processes high processivity and strand-displacement activity. The enzyme has been widely used in modern molecular biology for different applications such as amplification of plasmid templates for Sanger sequencing, virus genome sequencing using next-generation sequencing, genotyping, and whole genome amplifications such as amplification of viral genomes or human genome DNA for NGS.

We provide phi29 DNA polymerase and phi29 RCA DNA amplification kit. Both products can be used to amplify DNA at 30°C for 4 to 18 hours. The DNA template used for amplification can be plasmid DNA, lysate from a single colony or microliters of culture. The amplified product can be directly used as Sanger sequencing template without further cleanup.

Screening of correct mutants using single colony eliminates time for growing cultures and the DNA template is amplified by phi29 DNA polymerase for a few hours for Sanger sequenicng. The mutant can be identified faster without plasmid isolation.

Kit components:

ADS™ phi29 DNA polymerase comes with the enzyme and 10X Buffer; ADS™ phi29 RCA DNA amplification kit is a DNA amplification-ready kit that contains DNA lysate buffer, 2X Amplification buffer, and phi29 DNA polymerase.

Using PCR product as template?

If you are using PCR product as template for sequencing, visit the PCR and PCR Cleaning Beads page.

RCA amplification time course using phi29 DNA polymerase from a competition or AdvancedSeq (ADS)

 

 

 

 

 

 

 

 

ADS™ phi29 DNA Polymerase

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ADS™ phi29 RCA DNA Amplification Kit

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