Fragment Analysis

Fragment Analysis Products

ADS™ PCR and Purification Kit

High quality for PCR success and cleaning

TruPure™ Formamide

For high-performance sequencing sample resuspension

PwrPOP™ Polymers

For better signal intensity and stability

ADS™ 10x Sequencing Running Buffer

For cost-effective sequencing results

Fragment analysis refers to a genetic analysis that identifies the PCR fragment pattern difference by size among individual samples. It uses specifically designed and labeled PCR primers to generate PCR products from sample DNA. Then, specifically labeled PCR products mixed with fluorescent size standard are denatured and run on the capillary electrophoresis (CE) instrument to separate fragments by size. Different from Sanger sequencing in reaction mechanism, fragment analysis does not involve cycle sequencing reactions that generates nested extension products with one nucleotide difference; However,  similar to sequencing extension products, fluorescently labeled fragments are separated and detected through CE by size difference.

Applications for Fragment Analysis

Fragment analysis can be used in multiple areas including:

  • Single nucleotide polymorphorism (SNP) genotyping
  • DNA Fingerprinting
  • Microsatellite analysis
  • Microbial identification

Fragment Analysis Workflow and Products We Offer

The fragment workflow include DNA sample preparation, PCR fluorescent sample preparation, sample mixing and denaturing, and capillary electrophoresis and data analysis. We offer cost-effective products at different workflow steps for high-quality fragment analysis.

DNA Sample Prep
Labeled PCR Prep
ADS™ PCR and purification Kit
Sample Mixing & Denaturing
TruPure™ Formamide
Capillary Electrophoresis
PwrPOP™ P4/P7 Polymers
ADS™ 10x Running Buffer