Fragment Analysis

Fragment analysis refers to a genetic analysis that identifies the PCR fragment pattern difference by size among individual samples. It uses specifically designed and labeled PCR primers to generate PCR products from sample DNA. Then, specifically labeled PCR products mixed with fluorescent size standard are denatured and run on the capillary electrophoresis (CE) instrument to separate fragments by size. Different from Sanger sequencing in reaction mechanism, fragment analysis does not involve cycle sequencing reactions that generates nested extension products with one nucleotide difference; However,  similar to sequencing extension products, fluorescently labeled fragments are separated and detected through CE by size difference.

Applications for Fragment Analysis

Fragment analysis can be used in multiple areas including:

• Single nucleotide polymorphorism (SNP) genotyping
• DNA Fingerprinting
• Microsatellite analysis
• Microbial identification

Fragment Analysis Workflow and Products We Offer

The fragment workflow include DNA sample preparation, PCR fluorescent sample preparation, sample mixing and denaturing, and capillary electrophoresis and data analysis. We offer cost-effective products at different workflow steps for high-quality fragment analysis.