Dideoxy chain-termination Sanger sequencing has been used in the past 40 years as a gold standard for gene mutation analysis, de novo sequencing, resequencing, confirmation of next-generation sequencing (NGS), and gap filling for NGS. As a mature technology, Sanger sequencing will continue to play an important role in the DNA sequencing field.
The experimental workflow for Sanger sequencing includes:
Sanger Sequencing Products
High-performance alternative for BigDye™ Terminator v. 3.1 or 1.1 Cycling Sequencing Kit
Preparation of high-quality sequencing templates and primers for setting up sequencing reactions. PCR templates need to be purified or cleaned up to remove dNTPS, primers and DNA polymerase.
As a supplementary workflow step for instrument maintenance, regeneration of the capillary array to remove contaminants accumulated on the array after hundreds of CE runs. Regenerated array regains optimal performance.