SupreDye™ Cycle Sequencing Kits
As a powerful alternative to BigDye® Terminator v3.1 and v1.1 Cycle Sequencing kits, the SupreDye™ Cycle Sequencing Kits provide all the sequencing-required components, except for the template and primer, in a ready, pre-mixed format. Both single-stranded DNA or double-stranded DNA templates from PCR, plasmids, rolling circle amplified products, as well as G-rich templates (GT and GC) can be sequenced with high quality results.
SupreDye™ v1.1 and v3.1 Cycle Sequencing Kits are alternatives to BigDye® Terminator Cycle Sequencing v1.1 (for shorter reading or reading near primer) and v3.1 (for longer reading such as de novo sequencing and resequencing purpose), respectively. These products are used as “plug-and-play” substitute for BigDye® Terminator Cycle Sequencing kits without changing the protocol, volume, or setting. The products can be used with other products in the Sanger sequencing workflow either from AdvancedSeq or ABI.
The SupreDye™ dGTP v1.1 and v3.1 Cycle Sequencing Kits are designed for sequencing G-rich templates or templates with secondary structure such as hairpin with greater success. They are alternatives for dGTP BigDye® Terminator Cycle Sequencing v1.0 and v3.0, respectively. The SupreDye™ dGTP cycle sequenicng kits are used alone, without the need to mix with standard kits to reduce compression.
- Proven high-performance
- Widely accepted in community
- Increased robustness for difficult templates
- Even peak heights and long read lengths
The kits contain the SupreDye™ Cycle Sequencing mixture and 5x sequencing buffer.
“I had a chance last week to test your SupreDye Cycle Sequencing reagents. They performed very well with our existing protocol and no difference in results. A very satisfactory outcome. “—Technician at a university core lab
“I would like to tell you that we use SupreDye V3.1 and that works very well. I would like to quote the reagent to 1000 rxns.” —Lab Director at an international pharma company
“The dGTP kit has been doing the trick. I keep it my back pocket and use only when necessary. A huge plus is no spiking into the mix, just plug and play.” —Sequencing technologist at a US university
SupreDye Cycle Sequencing Kit (top) shows much better signal intensity and uniformity compared to Vendor T (bottom).
Compared to Vendor T product (bottom), SupreDye (top) chemistry shows much better performance for sequencing a difficult template with secondary structures by generating strong and long read signal.
SuperDye dGTP BD3 Cycle Sequencing Kit (top) provides much better performance on a GC-rich template than the regular BD3 Cycle Sequencing Kit (bottom).
SupreDye™ v1.1 Cycle Sequencing Kit
SupreDye™ v3.1 Cycle Sequencing Kit
SupreDye™ dGTP v1.1 Cycle Sequencing Kit
SupreDye™ dGTP v3.1 Cycle Sequencing Kit
ADS™ 5x Sequencing Reaction Buffer
ADS™ SupreDye™ Enhancer
Note: The products are for research use only, not for diagnostic procedures.