Blog
Sanger Sequencing of AAV Inverted Terminal Repeats Sequence as Part of the Quality Control in Viral Particle Production for Gene Therapy
Gene therapy The gene therapy concept started in 1960s but the first human clinical trial of gene therapy to correct a dysfunctional gene was conducted in 1990. Although early studies were hampered due to human death in clinical trials, gene therapy has only become a...
PwrPOP- 4,6, And 7 Polymers: Alternatives To POP-4,6, And 7 Polymers
During capillary electrophoresis, a particular type of polymer is injected into capillary array and used as liquid separation matrix for Sanger sequencing or fragment analysis. Currently, three types of Applied Biosystem (AB) polymers are used for these applications,...
How Does SupreDye Compare to BigDye?
AdvancedSeq developed the SupreDye chemistry as a drop-in-ready alternative to BigDye chemistry for high-quality Sanger sequencing. Not only for the dyes, the PwrPOP polymers, ADS 5Xsequencing buffer, ADS 10X running buffer, and TruPure formamide we carry are also...
Should I replace or regenerate my capillary array?
Capillary array as a carrier for polymers, the separation media, is critical for peak separation in Sanger sequencing and fragment analysis. Each capillary array costs several thousands of dollars; taking good care of the capillary array goes a long way for saving...
When Is Sanger Sequencing Used in Clinical Setting?
Sanger Sequencing and NGS Sanger sequencing as a gold standard for DNA sequencing is still commonly used in clinical settings. However, there is a trend that many clinical tests are using NGS to replace Sanger sequencing. The practice leverages the throughput,...
Sanger Sequencing for Screening and Confirmation of Mutations Generated from CRISPR Genome-editing
A simple introduction of CRISPR Clustered Regularly Interspaced Short Palindromic Repeat or CRISPR as a part of bacterial defense system against bacteriophage is used as a dominating gene-editing tool to make changes in targeted DNA sequences. The most popular...
Rolling Circle Amplification of Plasmid Template for Sanger Sequencing
Rolling circle amplification (RCA) Based on the natural rolling circle replication from bacterial plasmids, bacteriophage, and viruses, rolling circle amplification (RCA) technique was developed and widely used in molecular biology research applications including...
Purification Methods of Cycle Sequencing Reactions
Importance of cleaning cycle sequencing reactions In the sanger sequencing workflow, cycle sequencing reaction is certainly the core and critical step because the reaction determines if the extension products are appropriately synthesized and labeled with great...
BRAF V600E Enrichment Coupled Sanger Sequencing for Companion Diagnostics
As a gold standard, Sanger sequencing provides 99.99% accuracy and is the most commonly used sequencing method although NGS has dominated in the research field and is routinely used in many clinical labs. However, the limit in sensitivity has restricted the use of...
Prepare Purified PCR Templates for High-quality Sanger Sequencing
PCR product cleaning before Sanger sequencing PCR products are the most common template for Sanger sequencing to confirm the right sequence or detect a single or a few mutations. To achieve high-quality sequencing results, the PCR product must be a single product, a...
