by Wei Liu | Feb 16, 2022 | Blog
During capillary electrophoresis, a particular type of polymer is injected into capillary array and used as liquid separation matrix for Sanger sequencing or fragment analysis. Currently, three types of Applied Biosystem (AB) polymers are used for these applications,...
by Wei Liu | Nov 19, 2021 | Blog
Sanger Sequencing and NGS Sanger sequencing as a gold standard for DNA sequencing is still commonly used in clinical settings. However, there is a trend that many clinical tests are using NGS to replace Sanger sequencing. The practice leverages the throughput,...
by Wei Liu | May 9, 2022 | Blog
Gene therapy The gene therapy concept started in 1960s but the first human clinical trial of gene therapy to correct a dysfunctional gene was conducted in 1990. Although early studies were hampered due to human death in clinical trials, gene therapy has only become a...
by Wei Liu | Jan 27, 2022 | Blog
AdvancedSeq developed the SupreDye chemistry as a drop-in-ready alternative to BigDye chemistry for high-quality Sanger sequencing. Not only for the dyes, the PwrPOP polymers, ADS 5Xsequencing buffer, ADS 10X running buffer, and TruPure formamide we carry are also...
by Wei Liu | Jun 5, 2021 | Blog
PCR product cleaning before Sanger sequencing PCR products are the most common template for Sanger sequencing to confirm the right sequence or detect a single or a few mutations. To achieve high-quality sequencing results, the PCR product must be a single product, a...
by Wei Liu | Sep 24, 2021 | Blog
Rolling circle amplification (RCA) Based on the natural rolling circle replication from bacterial plasmids, bacteriophage, and viruses, rolling circle amplification (RCA) technique was developed and widely used in molecular biology research applications including...
Recent Comments