by Wei Liu | Oct 8, 2021 | Blog
A simple introduction of CRISPR Clustered Regularly Interspaced Short Palindromic Repeat or CRISPR as a part of bacterial defense system against bacteriophage is used as a dominating gene-editing tool to make changes in targeted DNA sequences. The most popular...
by Wei Liu | Sep 24, 2021 | Blog
Rolling circle amplification (RCA) Based on the natural rolling circle replication from bacterial plasmids, bacteriophage, and viruses, rolling circle amplification (RCA) technique was developed and widely used in molecular biology research applications including...
by Wei Liu | Sep 17, 2021 | Blog
Importance of cleaning cycle sequencing reactions In the sanger sequencing workflow, cycle sequencing reaction is certainly the core and critical step because the reaction determines if the extension products are appropriately synthesized and labeled with great...
by Wei Liu | Sep 1, 2021 | Blog
As a gold standard, Sanger sequencing provides 99.99% accuracy and is the most commonly used sequencing method although NGS has dominated in the research field and is routinely used in many clinical labs. However, the limit in sensitivity has restricted the use of...
by Wei Liu | Jun 5, 2021 | Blog
PCR product cleaning before Sanger sequencing PCR products are the most common template for Sanger sequencing to confirm the right sequence or detect a single or a few mutations. To achieve high-quality sequencing results, the PCR product must be a single product, a...
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