by Wei Liu | Sep 1, 2021 | Blog
As a gold standard, Sanger sequencing provides 99.99% accuracy and is the most commonly used sequencing method although NGS has dominated in the research field and is routinely used in many clinical labs. However, the limit in sensitivity has restricted the use of...
by Wei Liu | Sep 24, 2021 | Blog
Rolling circle amplification (RCA) Based on the natural rolling circle replication from bacterial plasmids, bacteriophage, and viruses, rolling circle amplification (RCA) technique was developed and widely used in molecular biology research applications including...
by Wei Liu | Jun 5, 2021 | Blog
PCR product cleaning before Sanger sequencing PCR products are the most common template for Sanger sequencing to confirm the right sequence or detect a single or a few mutations. To achieve high-quality sequencing results, the PCR product must be a single product, a...
by Wei Liu | Jan 27, 2022 | Blog
AdvancedSeq developed the SupreDye chemistry as a drop-in-ready alternative to BigDye chemistry for high-quality Sanger sequencing. Not only for the dyes, the PwrPOP polymers, ADS 5Xsequencing buffer, ADS 10X running buffer, and TruPure formamide we carry are also...
by Wei Liu | May 9, 2022 | Blog
Gene therapy The gene therapy concept started in 1960s but the first human clinical trial of gene therapy to correct a dysfunctional gene was conducted in 1990. Although early studies were hampered due to human death in clinical trials, gene therapy has only become a...
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