by Wei Liu | Nov 19, 2021 | Blog
Sanger Sequencing and NGS Sanger sequencing as a gold standard for DNA sequencing is still commonly used in clinical settings. However, there is a trend that many clinical tests are using NGS to replace Sanger sequencing. The practice leverages the throughput,...
by Wei Liu | Feb 16, 2022 | Blog
During capillary electrophoresis, a particular type of polymer is injected into capillary array and used as liquid separation matrix for Sanger sequencing or fragment analysis. Currently, three types of Applied Biosystem (AB) polymers are used for these applications,...
by Wei Liu | Apr 5, 2021 | Blog
Sanger sequencing data files can be used to measure copy number proportions A couple of months ago, we read the overlapping peaks of both the wildtype and the BRAF V600E mutant traces in the electropherogram of Sanger sequencing from a clinical sample. We wanted to...
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